Primer

Part:BBa_K4790052

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-08-14)


ASR1-L122K-F

The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 122nd amino acid from leucine to lysine. According to Codon preference in E.coli, AAA has the highest possibility coding lysine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None